Main author: Roberto de Armas
Summary: It was studied and determined the effect of carbohydrates of medium molecular weight (MMW) and soluble polysaccharides (SP) on the crystallization of sucrose, and it was corroborated the non-existence of SST activity (Sucrose-sucrose Fructose-Transferase) in juice, that is why its synthesis does not behave the way reported for typical fructans in C3 cycle plants. It was also isolated a fructanase from sugar cane's fresh juice, and it was established its purification method, which hydrolizes the SP and MMW, it was known that the enzyme is a protein of relatively low molecular mass, of alosteric behavior for both carbohydrates with more affinity for MMW. As enzyme it acts on the MMW and SP, there are produced fructose and galactitol; it was also reported for the first time in sugar cane with direct implications in crystallization and sucrose loss during the industrial process. It is also reported, for the first time, the existence of an enzyme that hydrolizes them and it is very specific, and there were created new perspectives for their possible industrial use.