(1858 - 1950)
Malacólogo y zoólogo. Eminente investigador y profesor universitario, discípulo de Felipe Poey. Su extensa obra comprende trabajos de Geología, Paleontología, Zoología, Arqueología e Historia. Su mayor aporte lo realizó en el conocimiento de la fauna fósil cubana.
Biomedical Sciences
1996 | Development of new analytic and preparative techniques that preserve biomolecules’ biological integrity
Main executives entity: Center for Biotechnology and Genetic Engineering
Main author: Eugenio Hardy Rando
Summary: The objective of this research was the development of some techniques that allow the non-destructive detection and the quantitative recovery of nucleic acids (DNA, RNA, oligonucleotides), lipopolysaccharides (LPS)/ lipooligosaccharides (LOS) and proteins that fulfill the requirements previously mentioned. The physicochemical principle of the detection methods here described lies on biomolecules' general ability to join the zinc ion and in the capacity of zinc and glyoxaline salts to form molecular complexes barely soluble in water. This scientific achievement consists on the development of: - New techniques for the reversible detection and recovery with high yields of DNA, RNA and oligonucleotides separated in polyacrylamide or agarose gels. These techniques conserve intact the biological activity and allow higher results than those obtained when these molecules are detected with the traditional technique of ethidium bromide. Unlike this last technique, the new technique doesn't use toxic reagents, harmful for the environment and the operator, and it doesn't cause damages on the molecules in study. - A new technique for the detection, with high sensibility, of lipopolysaccharides and lipooligosaccharides in polyacrilamide gels. It has been designed with the objective of being able to recover these biomolecules from gels and study their biological properties. A new method to separate proteins from polyacrilamide gels, which is compatible with the study of their biological activity and which works with very high yields even at trace levels (10-50 material ng, 1-5 picomoles). A new procedure to concentrate proteins contained in solutions diluted on the membranes used for automatic sequence, in order to determine the primary structure. This procedure, together with the above-mentioned method, has allowed obtaining sequences of very scarce natural proteins.
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