It consists on the fractionation of a preparation of the hepatitis B antigen purified in a preparative column of molecular exclusion with high resolution and the subsequent analysis of the fractions by a battery of physicochemical and immunochemical techniques. It is carried out an integral interpretation of the results, which allowed the authors discarding minority fractions of the protein with structural modifications that even when they don't affect its assembling and antigenicity, contribute to the molecular heterogeneity.

The most relevant scientific result was the identification of a heterogeneity source for the formation of supramolecular structures which are formed in the yeast cell. It should also be pointed out that these agregates are able to induce in mice a humoral answer superior to the native antigen's, and that possesses a promotion capacity of delayed hypersensitivity superior to the added antigen's, which constitutes a premise for the development of a new active immunotherapeutic agent in the treatment of chronic ways of hepatitis B. This work's results allow explaining the highest immunogenicity in Cuban vaccine due to its high content of added antigen.

It has 8 publications in magazines which have a great impact that endorse this result, and one of them was mentioned in the reference magazine ´´Current Awareness in Biomedicine: Oxygen Radicals´´ that selects the 70 most outstanding publications of the month from a source of 2500 scientific magazines.