This extensive and deep scientific work consisted on developing a somatic embryogeneses system in liquid media in sugarcane and its scaling in bireactor, getting encapsulated aclimatization-phased embryos converted, while evaluating, at field level, the possible generated genetic variability and its effect on the agricultural and industrial yield.

They decribe for the first time in the world, the stablishment of sugarcane embryogenic cell suspentions out of in vitro plants sections directly placed in liquid medium without a previous callus formation. This type of management reduces and shortens time for suspentions establishment in culture and the time exposed to auxins.

They create a new design for the bioreactor agitation and aeration systems folowing the requirements and the process of somatic embryogeneses of this culture, being also effective in other cultures. This design combines the use of a kind of paddle for agitating bubble-free aerating using a silicon hosepipe , decreasing thus cells and agreagated added to the system due to the design replacement.

Yields up to 32 700 total embryos per liter were achieved.

They established the endosperm mineral and organic composition, or artificial one in place.

23 200 plants directly obtained in bioreactors and germinated in culture bottles were climatized and taken to field in two provinces. Also 6000 encapsulated embryos plants were taken to seed banks to be evaluated in both provinces, proving their similarity to the ones obtained by classic in vitro methods and the ones obtained off cutting individual buds. This material kept its stability.

They have made 53 publications related to this result in different journals, most of them placed at the top level in the speciality.

These results are recorded in:

• - Chapters 10, 12 y 13 in the book "Propagación y mejora de las plantas por biotecnología" (Plants propagation and improvement through biotechnology) from theInstitute for Plants Biotechnology” (IBP) from the "Marta Abreu" Central University of Las Villas;
• - 6 doctorate thesis deffended, 2 of them at predeffense level and 3 master´s degree.
• - More than 39 presentations of works in scientific events.

they have been given several awards at provincial level and some others given by the Ministry of Higher (MES).Results are generalized.