The reference work is aimed to answer to the high present demand in vaccinology to have a variety of "carrier proteins" for the development of conjugated vaccines, which is a contribution to knowledge in this field.

The P64K protein, originally obtained through the recombinant method, had the potential risk of inducing autoantibodies against intramitochondrial proteins, due to its homology with antigens present in subcellular structures of mammals. For this reason, it was sought and obtained a new mutating variety to prevent this danger, and at the same time to conserve its versatility as protein carrier.

The impact of this work could be summarized in the elements provided by the immunologic characterization of P64K mutating protein, and in the demonstration that this is a protein molecule with versatile carrier and, at the same time, safe and immunogenic capacity to be used in healthy individuals without the risk of producing antimitochondrial autoantibodies. All these qualities are fundamental attributes for its use in prophylactic conjugated vaccines. It has 7 recent publications in prestigious magazines, 9 presentations in international and national events, as well as two diploma theses, and one in option to the Dr. of Pharmaceutical Sciences degree.