Riverside buffalos of Bufalypso race were introduced in Cuba from Panama and Trinidad-Tobago in 1983, with the aim of making good use of low coastal zones of our country, since this species is able in these conditions, where cattle can barely survive, of producing meat and milk in acceptable quantity. This work was aimed at studying the reproductive behaviour and the seasonal nature of this race, and the aplication of reproductive biotechnological methods, knowledge which is internationally limited, to extend and improve this species.

It was carried out in this work the hormonal characterization during the last trimester of gestation, establishing hormonal dynamics, and it was studied its relationship with the subsequent reproductive behavior. It was carried out the hormonal clinical study of puerperium in this race, being determined the signs and predominant clinical symptoms in the different puerperium stages, in both seasons of the year. Three rut detection methods were compared, being the most effective that of vasectomized buffalos, and there were established the most important features of estrus and estral cycle. It was recommended artificial insemination with detected natural estrus as the most economic method. It was developed a methodology in order to establish the gestation time under the conditions of cattle exploitations, based on the rectal clinical diagnosis in field and its corroboration with the help of the ultrasound scan.

All these scientific results constitute the first reports of Bufalypso race in the world. They are important for the country and the world since buffalo is becoming important for its contributions in proteins, its use possibilities in work and its economic upbringing. The work proposes methods to optimize the reproductive handling through rut induction, assisted copulation and artificial insemination. The introduction of these results is endorsed by 8 national production centers and the Pan-American Agricultural School of Honduras. The results of the work have been rewarded, presented in scientific events and published in 34 articles of scientific magazines (27 in prestigious international magazines) and in several contributions in 1 book.