The work is basically a study of the bactericidal and anticolonizing protector capacity of serum from voluntary human beings that received the candidate oral vaccinal dose with the genetically attenuated 638stump. The protector capacity was evaluated in two models of different animals. There were also purified IgG and IgA antibodies and it was evaluated the biological activity of these purified antibodies.

The study was extended to the purification of different V. cholerae antigens, which were used to characterize the specificity of antibodies in serum; as well as to identify the most important antigens for the protector activity facing wild stumps of different biotype and serotype from O1 serogroup and to a O139 serogroup stump.

These results extend the characterization of the immunogenic effect of 638 vaccinal candidate, which is a Cuban and patented original product. There are identified the main antigens identified by the seric answer and it is described the vibriosis and anticolonizing activities of serums for the first time. Also, the identification of the changes in the protector activity when the serums face bacteria of different serotype, biotype and serogroup, allows pointing out those antigens that should be included in the formulation of a future subunits' vaccine. The results are published in the Vaccíne magazine and in applied biotechnology; and it was the content of a doctoral thesis.