The presented work essentially consists on Pichia pastoris clonning and expression of the gene encoding E glycoprotein in Dengue 4 virus casing and the evaluation of its protecting immunogenicity and induction in mice and non-human primates. This protein is the responsible of the virus-cell link, and also induces virus neutralizing antibodies and viral amplification mediating antibodies.

The work´s sicnetific novelty consits on the expression of a recombinant E protein truncated by its terminal carboxyl portion with a modified glycosilation. It is however capable of generating protection against Dengue 4 virus in mice and non-human primates. This protection is correlated to neutralizing antibodies induction and a cellular immunoresponse, what is for the first time reported in the international scientific community.

There is an international patent request and 4 publications in foreign journals of impact, which denotes international acceptance of its contribution to the efforts in develooping a safe and effective vaccine against Dengue.